Re-evaluation of EDTA-chelated biuret reagent.
نویسندگان
چکیده
We studied the optimal composition of EDTA-chelated biuret reagent for the determination of protein in serum. A reagent containing 18 mmol of EDTA, 15 mmol of Cu2, and 1 mol of NaOH per liter exhibits a very low blankbut about the same sensitivity as tartrate-chelated reagents. Maximum color intensity is attained within 25 mm at room temperature, and adheres to Beer’s law up to 120 g of protein per liter.
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ورودعنوان ژورنال:
- Clinical chemistry
دوره 20 10 شماره
صفحات -
تاریخ انتشار 1974